Philip H. Jones, Onofrio M. Maragò & Giovanni Volpe
Fig. 8.6 — Sample preparation
Sample preparation: (a) a glass slide is carefully cleaned; (b) two stripes of parafilm (about 100 μm thick) are placed on the glass; (c) a coverslip is placed on top of the parafilm, and the sample is heated in order for the parafilm to stick to the glass surfaces (not shown); (d) the excess parafilm is removed; (e) the chamber is filled with sample solution (e.g., microparticles suspended in an aqueous solution) and is sealed using vacuum grease; (g) the chamber is placed upside down on the sample stage and is gently fixed with some plastic screws.